Looking for a career that challenges and inspires? Lawrence Livermore National Laboratory is hiring for a nuclear facility engineer, systems design and testing engineer, and a senior scientific technologist, along with many other roles in science, technology, engineering, and beyond. At the lab, every role contributes to ground-breaking projects in national security, advanced computing, and scientific research. All within a collaborative mission-driven environment, discover open positions at llnl.gov forward slash careers, where big ideas come to life. August 21, 2013. Damascus, Syria. In the Pridon darkness, families huddled in basements, seeking shelter from what they thought was just another night of shelling in the country's brutal civil war. But the rockets that fell on eastern Guta that morning carried something different. Something invisible. Something that would turn their underground refuge into a death trap. You could hear the sound of the rocket in the air, but you could not hear any sound of explosion. One witness told the Guardian, no blast, no shrapnel, no fire, just silence. And then people started having trouble breathing. Because it was heavier than air, the deadly gas seeped down into those basements where people had taken shelter, and hundreds of people died. 85% of blood samples from the attack site tested positive for seren. Thousands of miles away in northern California, scientists at llnll live for more national laboratories forensic science center were thinking about how they might help answer the crucial questions. Who was responsible for the chemical attack? And how can we prevent another tragedy like this? First question is always is there a chemical war for agent present? If the answer is yes, which in the case of Syria it was, then it's what other information can we get from that? From this chemical information that you have, can you tell where this came from? Which side of a conflict used the weapon? Who's material did this come from? What that really relies upon is some ground truth. In the world of chemical forensics, every molecule tells a story. Every impurity is a clue. Truth that creates justice for the past, and safety for the future. Welcome to the Big Ideas Lab, your exploration inside Lawrence Livermore National Laboratory. Here untold stories meet boundary pushing pioneers and get unparalleled access inside the gates. From national security challenges to computing revolutions, discover the innovations that are shaping tomorrow today. Join a team where expertise makes a difference. Lawrence Livermore National Laboratory is hiring for a nurse practitioner, physician assistant, a senior health physicist, and a laser modeling physicist. And the list of open positions doesn't end there. There are more than 100 job openings across science, engineering, IT, HR, and the skilled trades. This is more than a job. It's an opportunity to help shape the future. Explore all open positions and start your next career adventure today at llll.gov forward slash careers. That's llll.gov forward slash careers. We're the scientists at work in the shadows. If we're doing our job well, you don't hear about it. Audrey is the director of the FSC at Lawrence Livermore National Laboratory. The forensic science center has been around for a little over 30 years, and our mission is really twofold. We function as an operational forensic science lab. We can get case work from local law enforcement, state law enforcement, federal law enforcement. That's what we focused on in the last episode, with cases like a mysterious seemingly toxic woman, a mortuary scheme, and even the unibomber. But here in part two, we're talking about the second prong of the FSC. We do research and development, cutting edge, developing new techniques related to counterterrorism for weapons of mass destruction. It's a pursuit of both national and global security. And as a member of the designated laboratory network that supports the OPCW, the organization for the prohibition of chemical weapons, the forensic science center has a major role in investigating suspected chemical weapon use, like in Damascus. That was the case in Syria, about 10 years ago, both Seren and as well as Silphermustard were used. These, along with other nerve agents, Rick Kruehl-Havik on the human body. They work by interrupting brain signals before they can reach vital organs, essentially paralyzing many automatic body activities, including digestive and lung function. Many die from asphyxiation because they simply stop breathing. After an investigation by the OPCW, the United Nations took swift action. Together, the world, with a single voice for the first time, is imposing binding obligations on the Assad regime. A resolution was passed later that year, demanding Syria's complete dissolution of their chemical weapons program. This was a measure that aimed to prevent another catastrophe, because the suffering these chemicals cause is nearly incomprehensible. Of course, if your only exposure to chemical weapons comes from Hollywood, you might have a very different picture of what we're talking about. Take the rock, a 1996 action thriller about chemical weapons on Alcatraz Island. The movie gets some details about the effects right, but not everything. It's a colonel Surrey's inhibitor. It stops the brain from sending nerve messages down the spinal cord within 30 seconds. Carlos Valdez, one of the leading chemists at the FSC, knows firsthand the difference between real-life chemical agents and those in the film. Really all against string of pearls configuration. Unfortunately, incredibly unstable. The X is not glowing green material. It's actually a clear tasteless liquid or vapor. And to counteract its effects. Injective in your heart before you zoom-mails. First of all, you don't stab yourself in the heart. You just do it in your thigh on your leg. That's an atropine shot, which functions as an antidote to some nerve agents. The movie got the drugs name right, but not its method of injection. And in real life, it's more often a precautionary measure than a solution. The chemists at the FSC think those treatments can be strengthened and improved. My projects have been more developing antidotes. They're more efficient for some of those chemical agents that are coming through, talking about Seren, you talking about VX. That's what the research part comes into finding ways of destroying them more efficiently. At the end of the day, a chemical warfare agent is an organic chemical. And so a lot of the methods and capabilities that we use for those types of identification we can use for other things as well. The FSC studies a wide spectrum of threats. They deal with anything biological or chemical, radioactive substances, and explosive and nuclear materials. In short, anything. That makes the FSC one of a kind. The forensic science center is the only place in the US that could accept a truly mixed hazard sample. So theoretically, if there was a biological material, a chemical agent, an explosive, radiological, and nuclear material, all mixed in one, our detection limit depends on what the compound is. We are commonly down in the parts per trillion. So one piece of the molecule that we're detecting in one trillion other molecules. If we know exactly what we're looking for, sometimes even lower. But the FSC also works to study and understand the makeup of unknown samples. You don't know what you have until you've identified it. If the reference data doesn't exist, we can make some of that on the fly to help us answer the questions that we need to answer. With my background synthesis, I help out in projects that have to do with the identification of unknown compounds. They come up with a structure and they tell me Carlos is this structure feasible. And if I say no, it's not, then they go back to their analysis and they try to come up with another one. You need to do the experiments to figure out what's going on. You need to have the real material in your hands. That's the other reason we have the two missions, our operational sample analysis and our R&D mission. That kind of weave back and forth like this because we can also do that synthesis. You need to test them. That's the bottom line. If you don't have them, you can test the stuff you're making to destroy them or to help people out. One of Carlos's main projects takes the same techniques used to evaluate and fight nerve agents and applies them to a different substance. Fentanyl. Join a team where expertise makes a difference. Lawrence Livermore National Laboratory is hiring for a nurse practitioner, physician assistant, a senior health physicist, and a laser modeling physicist. And the list of open positions doesn't end there. There are more than 100 job openings across science, engineering, IT, HR, and the skilled trades. This is more than a job. It's an opportunity to help shape the future. Explore all open positions and start your next career adventure today at llnl.gov-careers. It's a man-made synthetic opioid that's 100 times stronger than morphine. It's odorless, tasteless, and an amount equal to a few grains of salt is enough to kill. It's the main drug in today's opioid overdose epidemic. Fentanyl. Fentanyl is a drug that once it enters your body, it binds a receptor known as mu opioid receptor. This receptor mediates signals that your nervous system sends to various parts of your body. For example, respiration. So your brain sends a signal to your muscles in your respiratory tract, in your ribcage, to in unison, breathe. The problem with this is that this is a very highly regulated event. Your body actually creates its own natural opioids, not fentanyl, in small amounts. They join and separate from different nervous system receptors and are able to operate your internal functions like a light switch, depending on what the brain requests. Fentanyl, what it does is it goes in and binds that receptor and it locks it in place. Meaning when the signal goes out to say, okay, contract the muscles, then it cannot send the signal for muscles relaxed now. You're going to start having trouble breathing. But some of it is also going to still be circulating in your blood, meaning that even if those come off from the receptors, you still have a lot of it that can find lock in place and basically kill you by suffocation. The most common treatment for a fentanyl overdose is naloxone, commonly known as Narcan. What it does is goes in and binds the receptor, but it comes off quickly. It doesn't stay in there, but it is that dynamic in and out, in and out, in and out that prevents the fentanyl from going in and locking in. Basically, it ensures that the light switch can't get stuck in one position. Now, the problem is that like I said, fentanyl can stay in your body for up to like nine hours. Naloxone only stays for about four. So you need to get those suggest of Naloxone every other two hours to keep you going in the fight until the fentanyl you eliminate it from your body. So Naloxone is a great antidote. It works really well, but is there a way that we can actually accelerate, for example, the elimination of fentanyl out of the body or neutralize it until it gets eliminated. So it doesn't keep coming back. A treatment like that would be revolutionary. It would be a significant victory in the opioid epidemic battle and save countless lives. But can it be done? So the work with fentanyl started back in 2014. My idea was to see if we can use compounds, not a cyclo dexperience, like donut shaped molecules. And I was thinking maybe we can use them to trap fentanyl in their interior. So in the donut, the fentanyl will sit in and we can hopefully neutralize it. Carlos and his team worked for three years to find a viable molecule. And in 2017, they did. We found a candidate that was able to bind fentanyl and trap it really well. After that, the defense and threat reduction agency funded the project for an additional three years. The FSC used that time to refine and test the antidote before human trials. But it's not just a more powerful type of naloxone. This can be used as a preventative measure before exposure. We're developing what we call medical countermeasures. The idea was to have a compound that you can inject yourself and circulates in your body and it just provides a layer protection so that if you get hit with fentanyl, this is already in your body, monitoring things. This is to give you an extra layer protection prior to going into a place where you might be exposed to fentanyl. The project is currently seeking approval from the FDA for distribution. After some more studies to ensure it's safe for human use, this treatment could be used in countless ways, including by first responders during emergency services. Paramedics respond into a call where somebody is showing signs of opioid poisoning, right? So at a point, you want to protect them from becoming exposed to it by grabbing the person and trying to carry them into the ambulance for something like that. The treatment is cheap and easy to make, and its uses go beyond accidental overdoses and exposures. At the stage from the auditorium jumps a man in a military cloth and with a Kalashnikov gun. In 2002, Chechen rebels took over a theater in Moscow holding over 700 people hostage. Russian special forces responded by pushing fentanyl through the vents. And it's the first time that I know off that fentanyls were used as an actual weapon. We're fentanyl to be used as a weapon again, and antidote like carlosis could be vital to a military response. So in addition to helping civilian response teams, the project is a national security measure. But FSC's innovations don't just protect soldiers and spies. They've made their way into the hands of local law enforcement, fire departments, and even private citizens. Take, for example, a simple swab test they developed to identify explosive material. The Elite Kit is an acronym easy to live a more inspection test for explosives. It's like a little swab that you could rub any surface with that you had a suspected explosive contaminant on it. You put that back into the kit and break these two little ampules looking for a color change in the piece of paper if an explosive is present. So it can give that easy indicator of is there an explosive present on this surface? If you see a white powder, is it an explosive? You can get that quick information that may help first responders with entry to a scene. It's commercially available. You could go online and buy your very own Elite Kit if you wanted one. Explosives, fentanyl, and chemical warfare agents seem like very obvious threats. But sometimes the FSC discovers insidious chemicals hiding in plain sight. Just started maybe six, eight months ago looking into pesticides that are being used on marijuana crops. They're cheap. They're easy to get their hands on. Unfortunately, they're also really hazardous. If you're buying marijuana, you want to know that it doesn't have these pesticides in it. Even if you don't personally smoke or ingest THC products, the pesticides used in its growth could still get into the water supply and affect say the strawberries you buy at the farmer's market. And looking at those health implications, environmental implications, implications in the water as you know all this runs off or unfortunately affects the neighbor's crops too. The problem is a lot of these illegal pesticides are imported and unstudied. We're doing some research here to determine what are those hazards. What are these actual pesticides? That's sort of step one, getting a bag of pesticide unlabeled off the internet. What does it actually contain? And then the next step is we're going to start exposing marijuana plants to these pesticides. That way we're able to look at how any of those pesticides remain on the plant, how easily can they be removed from the plant? And are they actually leaving signatures within the plant? If they're actually being incorporated into the plant, then that's something that people should know. This investigation came to the lab through the California EPA as they were concerned about the use of these illegal and uncharacterized substances. But the FSC finds its projects from multiple sources, including law enforcement, special government request, and even in-house suggestions. It's one amazing thing about the center, and Lawrence Livermore as a whole. If you're passionate about solving a particular problem, you can probably do it. One of the good things working in this lab is that we have an internal process for funding ideas called the LDRD. That stands for lab directed research and development. It's what funded Carlos's current work, a preventative measure for opioid overdoses, one of the leading causes of death in the United States. And because the projects the FSC takes on are driven both by necessity and passion, it makes for an ever-changing, always exciting work environment. It's not just science for the sake of science. There's names and faces on the end of it of people that we're actually helping. It's a great feeling. I think that's what gives me always smiling and loving the job that I do. Everyone wants to make the world a better place, keeping a world free of chemical weapons or aspiring to have a world free of chemical weapons is a great goal to be working towards. It's a massive undertaking, but with the entirety of Lawrence Livermore National Lab at their disposal, the FSC, Audrey, Carlos, and their colleagues have access to experts in any scientific field you can think of. We always say we've got 9,000 co-workers around us here that we can pull in as needed. So anytime there's any forensic expertise needed, we likely have it at the lab. If it's not already within our center, it's here on site and we can pull those people in right away. When we put our heads together, we can do great things. It's the type of job everyone dreams about. It's never the same thing twice. You never know what's going to come through the door. Every night, basically, that's when my brain is going. It's always working on solutions, things that we can do. I write it down in an old pattern next to my bed and if I go to work, fall in mourning and try these things out. Never boring. A great support system and the opportunity to make a real difference. We all want to leave some sort of legacy. We all want to matter the work we do at the FSC matters. Thank you for tuning in to Big Ideas Lab. If you loved what you heard, please let us know by leaving a rating and a review. And if you haven't already, don't forget to hit the follow or subscribe button in your podcast app to keep up with our latest episode. Thanks for listening.
